Anticorps Polyclonal de lapin anti-Collagen Type XI

Collagen Type XI Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 21841-1-AP

Synonymes

COL11, COL11A1, CO11A1, COLL6, Collagen alpha 1(XI) chain

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Galerie de données de validation

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  WB analysis using 21841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 21841-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21841-1-AP (Collagen Type XI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 21841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Collagen Type XI antibody (21841-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules U2OS,
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 5 publications below
• IF: See 4 publications below

Informations sur le produit

21841-1-AP cible Collagen Type XI dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type XI Protéine recombinante Ag16509
• Nom complet: collagen, type XI, alpha 1
• Masse moléculaire calculée: 1818 aa, 182 kDa
• Poids moléculaire observé: 250 kDa, 181 kDa
• Numéro d’acquisition GenBank: BC117697
• Symbole du gène: COL11A1
• Identification du gène (NCBI): 1301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COL11A1 is an important component of collagen XI and is considered to play an important role in a variety of connective tissue diseases. Type XI collagen is widely distributed in tissues including cartilage, bone, and muscle, and its hereditary absence in Marshall's syndrome, Stickler's syndrome, fibrochondrogenesis, and nonsyndromic hearing loss deafness, autosomal dominant 37 leads to facial and eye abnormalities, hearing loss, and joint problems. The COL11A1 expression was significantly correlated with the degree of immune infiltration and the expression of a variety of immune cell markers in tumor tissue. (PMID: 36160004 33610546)

Publications

Species	Application	Title
human	IF	Transl Oncol; Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer; Authors - Nanxin Zheng
human	IHC	J Orthop Res; Increased Wnt5a/ROR2 signaling is associated with chondrogenesis in meniscal degeneration; Authors - Yusuke Inoue
human	IHC,IF	BMC Med Genomics; Analysis of cancer-associated fibroblasts related genes identifies COL11A1 associated with lung adenocarcinoma prognosis; Authors - Haosheng Zheng
human	IF	Cancer Res; Dissecting FAP+ Cell Diversity in Pancreatic Cancer Uncovers an Interferon-Response Subtype of Cancer-Associated Fibroblasts with Tumor-Restraining Properties; Authors - Joshua Cumming
human	IHC	Oral Oncol; Single-cell transcriptomics reveal the effects of chemoimmunotherapy on hypopharyngeal cancer and its tumor microenvironment; Authors - Xiaoli Zhu
human	IHC,IF	Oncogene; Single-cell landscape of undifferentiated pleomorphic sarcoma; Authors - Yifei Lu

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