Anticorps Polyclonal de lapin anti-Collagen Type XXV

Collagen Type XXV Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12738-1-PBS

Synonymes

COL25, COL25A1, Alzheimer disease amyloid-associated protein, CLAC-P, COL 25A1

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Galerie de données de validation

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  WB analysis using 12738-1-AP (same clone as 12738-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12738-1-AP (Collagen Type XXV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12738-1-AP (same clone as 12738-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 12738-1-AP (same clone as 12738-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12738-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12738-1-PBS cible Collagen Type XXV dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type XXV Protéine recombinante Ag3634
• Nom complet: collagen, type XXV, alpha 1
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC036669
• Symbole du gène: COL25A1
• Identification du gène (NCBI): 84570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Collagen alpha-1(XXV) chain is a protein that in humans is encoded by the COL25A1 gene. COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease brains.(PMID: 11927537)

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