Anticorps Polyclonal de lapin anti-Collagen Type XXVII

Collagen Type XXVII Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15673-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15673-1-AP (same clone as 15673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15673-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 15673-1-AP (same clone as 15673-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15673-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15673-1-PBS cible Collagen Type XXVII dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type XXVII Protéine recombinante Ag8140
• Nom complet: collagen, type XXVII, alpha 1
• Masse moléculaire calculée: 1860 aa, 87 kDa
• Poids moléculaire observé: 68-73 kDa
• Numéro d’acquisition GenBank: BC007696
• Symbole du gène: COL27A1
• Identification du gène (NCBI): 85301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Collagen Type XXVII, also named COL27A1, it is a member of the collagen fibrillar gene family . It is located in the extrcellular matrix. The calculated molecular weight of COL27A1 is 186 kDa. This antibody can recognize the 73 kDa isoform of target. The high level of Col27a1 expression in mouse cartilage suggested the possibility that Col27a1 may be regulated in cartilage by SOX9, as are Col2a1, Col11a2, Col9a1, and Col9a2 (PMID: 15922909). Expression analyses of the mouse col27a1 gene demonstrated high expression in cartilage, the eye and ear, but also in lung and colon. It is likely that the major protein product of COL27A1, proa1(XXVII), is a component of the extracellular matrices of cartilage and these other tissues. Study of this collagen should yield insights into normal chondrogenesis, and provide clues to the pathogenesis of some chondrodysplasias and disorders of other tissues in which this gene is expressed (PMID: 12714037).

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