Anticorps Polyclonal de lapin anti-Collagen Type XXVIII

Collagen Type XXVIII Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24401-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 24401-1-AP (same clone as 24401-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24401-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 24401-1-AP (same clone as 24401-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24401-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24401-1-PBS cible Collagen Type XXVIII dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type XXVIII Protéine recombinante Ag21459
• Nom complet: collagen, type XXVIII, alpha 1
• Masse moléculaire calculée: 1125 aa, 117 kDa
• Poids moléculaire observé: 72 kDa, 100-117 kDa
• Numéro d’acquisition GenBank: BC136892
• Symbole du gène: COL28A1
• Identification du gène (NCBI): 340267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

COL28A1 (collagen type XXVIII alpha 1 chain), also known as COL28. It is expected to be located in the basement membranes, cytoplasm and nucleus. And the protein is mainly expressed in salivary gland and adrenal. This antibody can recognize the 117 kDa and 72 kDa isoforms of target. COL28A1 is a new collagen that cannot clearly be assigned to any collagen subgroup, despite the fact that it contains VWA domains. Ongoing investigations will shed further light on the role of COL28A1 in the assembly of nerve fiber basement membranes. Although no human mutation has been associated with COL28A1, it is tempting to speculate that mutations in this gene would lead to neurodegenerative disease (PMID: 16330543).

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