Anticorps Polyclonal de lapin anti-Complement factor B

Complement factor B Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10170-1-PBS

Synonymes

CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD

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Galerie de données de validation

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  WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10170-1-AP (same clone as 10170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  WB analysis of human blood using 10170-1-AP (same clone as 10170-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 10170-1-AP (same clone as 10170-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10170-1-AP (same clone as 10170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 10170-1-AP (same clone as 10170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10170-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10170-1-PBS cible Complement factor B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Complement factor B Protéine recombinante Ag0226
• Nom complet: complement factor B
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 93-100 kDa, 60-69 kDa
• Numéro d’acquisition GenBank: BC007990
• Symbole du gène: Complement factor B
• Identification du gène (NCBI): 629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

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