Anticorps Recombinant de lapin anti-Complement factor B

Complement factor B Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241141A8

N° de cat : 84101-5-PBS

Synonymes

CFB, CFAB, C3/C5 convertase, BFD, BF

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Galerie de données de validation

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  WB analysis using 84101-5-RR (same clone as 84101-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84101-5-RR (Complement factor B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84101-5-RR (same clone as 84101-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84101-5-RR (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84101-5-RR (same clone as 84101-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor B antibody (84101-5-RR, Clone: 241141A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84101-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84101-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84101-5-RR against Human Complement factor B were performed. The affinity constant is 2.27 nM.

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Informations sur le produit

84101-5-PBS cible Complement factor B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Complement factor B Protéine recombinante Ag0226
• Nom complet: complement factor B
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 93-100 kDa
• Numéro d’acquisition GenBank: BC007990
• Symbole du gène: Complement factor B
• Identification du gène (NCBI): 629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease that is associated with C3b and forms the alternative pathway for C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

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