Anticorps Recombinant de lapin anti-Connexin 43

Connexin 43 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3O6

N° de cat : 80543-1-PBS

Synonymes

Connexin-43, GJA1, 3O6, CX43, Gap junction alpha 1 protein

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Galerie de données de validation

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  WB analysis using 80543-1-RR (same clone as 80543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80543-1-RR (same clone as 80543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80543-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80543-1-RR against Human Connexin 43 were performed. The affinity constant is 5.19 nM.

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Informations sur le produit

80543-1-PBS cible Connexin 43 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Connexin 43 Protéine recombinante Ag25533
• Nom complet: gap junction protein, alpha 1, 43kDa
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC026329
• Symbole du gène: Connexin-43
• Identification du gène (NCBI): 2697
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

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