Anticorps Recombinant de lapin anti-Cortactin

• Phare
• Validé par KD/KO

Cortactin Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241987A12

N° de cat : 84569-1-PBS

Synonymes

CTTN, SRC8, EMS1, 241987A12

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Galerie de données de validation

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  WB analysis using 84569-1-RR (same clone as 84569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84569-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 84569-1-RR (same clone as 84569-1-PBS)

  WB result of Cortactin antibody (84569-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and Cortactin knockout HAP1 cells. This data was developed using the same antibody clone with 84569-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01412-2

  Sandwich ELISA standard curve of MP01412-2, Human Cortactin Recombinant Matched Antibody Pair - PBS only. 84569-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1940. 84569-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01412-2

  The mean Cortactin concentration was determined to be 848.3 ng/mL in A549 cell extract based on a 2.3 mg/mL extract load, 581.1 ng/mL in HEK-293 cell extract based on a 3.3 mg/mL extract load and 493.0 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP01412-2

  Cytometric bead array standard curve of MP01412-2, Cortactin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84569-2-PBS. Detection antibody: 84569-1-PBS. Standard: Ag1940. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84569-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

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Informations sur le produit

84569-1-PBS cible Cortactin dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cortactin Protéine recombinante Ag1940
• Nom complet: cortactin
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC008799
• Symbole du gène: Cortactin
• Identification du gène (NCBI): 2017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

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