Anticorps Recombinant de lapin anti-Cortactin

• Phare
• Validé par KD/KO

Cortactin Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241987A12

N° de cat : 84569-1-RR

Synonymes

CTTN, SRC8, EMS1, 241987A12

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Galerie de données de validation

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  WB analysis using 84569-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84569-1-RR (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 84569-1-RR

  WB result of Cortactin antibody (84569-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and Cortactin knockout HAP1 cells.

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  Affinity and Kinetic Characterization of 84569-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84569-1-RR against Human Cortactin were performed. The affinity constant is 7.44 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

84569-1-RR cible Cortactin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cortactin Protéine recombinante Ag1940
• Nom complet: cortactin
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC008799
• Symbole du gène: Cortactin
• Identification du gène (NCBI): 2017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

Publications

Species	Application	Title
human	WB	Cell Rep Med; Integrative proteomic profiling of tumor and plasma extracellular vesicles identifies a diagnostic biomarker panel for colorectal cancer; Authors - Jun Wang

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