Anticorps Polyclonal de lapin anti-Cryptochrome 1

• Phare
• Validé par KD/KO

Cryptochrome 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13474-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13474-1-AP (same clone as 13474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13474-1-AP (same clone as 13474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13474-1-AP (same clone as 13474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13474-1-AP (same clone as 13474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody (13474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13474-1-AP (same clone as 13474-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 13474-1-AP (same clone as 13474-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13474-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13474-1-PBS cible Cryptochrome 1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cryptochrome 1 Protéine recombinante Ag4270
• Nom complet: cryptochrome 1 (photolyase-like)
• Masse moléculaire calculée: 586 aa, 66 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC030519
• Symbole du gène: CRY1
• Identification du gène (NCBI): 1407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.

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