Anticorps Recombinant de lapin anti-CXCL1

CXCL1 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240799D7

N° de cat : 83745-2-PBS

Synonymes

Cinc1, CINC-1, C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, Gro

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00698-3

  Sandwich ELISA standard curve of MP00698-3, Rat Cxcl1 Recombinant Matched Antibody Pair - PBS only. 83745-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0948. 83745-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL

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  Sample test of MP00698-3

  Rat lung (1 lung, 1-2 mm pieces) were cultured for 3 days in RPMI1640 plus 15% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 465.9 pg/mL; Rat spleen (1/2 spleen; 1-2 mm pieces) were cultured for 3 days in 50 mL DMEM supplemented with 10% fetal bovine serum and stimulated with 5 μg/mL LPS. An aliquot of cell culture supernatants was removed, The cell culture supernatants was assayed for rat Cxcl1 and measured 17,149.5 pg/mL.

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  Sample test of MP00698-3

  Serum of sixteen rats was measured. The rat Cxcl1 concentration of detected samples was determined to be 357.1 pg/mL with a range of 143.6 - 694.0 pg/mL.

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  Affinity and Kinetic Characterization of 83745-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 83745-2-PBS against Rat CXCL1 were performed. The affinity constant is 17.2 nM.

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Informations sur le produit

83745-2-PBS cible CXCL1 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CXCL1 Protéine recombinante Eg0948
• Nom complet: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Masse moléculaire calculée: 10 kDa
• Numéro d’acquisition GenBank: NM_030845.1
• Symbole du gène: Cxcl1
• Identification du gène (NCBI): 81503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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