Anticorps Polyclonal de lapin anti-Cyclin A1

Cyclin A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13295-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13295-1-AP (same clone as 13295-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13295-1-AP (Cyclin A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13295-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13295-1-AP (same clone as 13295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13295-1-AP (Cyclin A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13295-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13295-1-AP (same clone as 13295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13295-1-AP (Cyclin A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13295-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13295-1-PBS cible Cyclin A1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin A1 Protéine recombinante Ag4005
• Nom complet: cyclin A1
• Masse moléculaire calculée: 464 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC036346
• Symbole du gène: Cyclin A1
• Identification du gène (NCBI): 8900
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNA1 binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. CCNA1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. In general, the expression of CCNA1 is tissue-specific and high CCNA1 expression is limited to testis; besides, lower levels of CCNA1 expression are also observed in other human cell lines and in healthy brain. Currently, CCNA1 expression has been illustrated to be downregulated in various tumors, including head and neck squamous-cell cancer (HNSCC) and nasopharyngeal carcinoma; and the promoter of the CCNA1 gene is found to be frequently methylated in colon cancer and HNSCC.

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