Anticorps Recombinant de lapin anti-Cyclin A1

Cyclin A1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250550D7

N° de cat : 86151-1-RR

Synonymes

CCNA1, Cyclin A, Cyclin-A1

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Galerie de données de validation

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  WB analysis of SKOV-3 using 86151-1-RR

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 86151-1-RR (CCNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86151-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86151-1-RR (CCNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86151-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86151-1-RR against Human Cyclin A1 were performed. The affinity constant is 0.397 nM.

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, tissu testiculaire de rat, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86151-1-RR cible Cyclin A1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cyclin A1 Protéine recombinante Ag4005
• Nom complet: cyclin A1
• Masse moléculaire calculée: 464 aa, 52 kDa
• Poids moléculaire observé: 52-55 kDa
• Numéro d’acquisition GenBank: BC036346
• Symbole du gène: Cyclin A1
• Identification du gène (NCBI): 8900
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNA1 binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. CCNA1 was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. In general, the expression of CCNA1 is tissue-specific and high CCNA1 expression is limited to testis; besides, lower levels of CCNA1 expression are also observed in other human cell lines and in healthy brain. Currently, CCNA1 expression has been illustrated to be downregulated in various tumors, including head and neck squamous-cell cancer (HNSCC) and nasopharyngeal carcinoma; and the promoter of the CCNA1 gene is found to be frequently methylated in colon cancer and HNSCC.

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