Anticorps Monoclonal anti-Cyclin A2

• Phare
• Validé par KD/KO

Cyclin A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C6C11

N° de cat : 66391-1-PBS

Synonymes

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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Galerie de données de validation

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  WB analysis using 66391-1-Ig (same clone as 66391-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

  WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells. This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66391-1-Ig (same clone as 66391-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66391-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66391-1-PBS cible Cyclin A2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin A2 Protéine recombinante Ag12765
• Nom complet: cyclin A2
• Masse moléculaire calculée: 432 aa, 49 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC104783
• Symbole du gène: Cyclin A2
• Identification du gène (NCBI): 890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

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