Anticorps Polyclonal de lapin anti-Cyclin B1

• Phare
• Validé par KD/KO

Cyclin B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

56

N° de cat : 28603-1-AP

Synonymes

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

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Galerie de données de validation

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  WB analysis of HeLa using 28603-1-AP

  WB result of Cyclin B1 antibody (28603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.

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  WB analysis using 28603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28603-1-AP (Cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28603-1-AP

  IP result of anti-Cyclin B1(IP:28603-1-AP, 4ug; Detection:28603-1-AP 1:2000) with HeLa cells lysate 1800 ug.

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  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HT-29 using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules C6, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 53 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

28603-1-AP cible Cyclin B1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, bovin, canin, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin B1 Protéine recombinante Ag29426
• Nom complet: cyclin B1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC006510
• Symbole du gène: CCNB1
• Identification du gène (NCBI): 891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes; Authors - Jian Chen
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB	Mol Oncol; Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma; Authors - Mayu Nakagawa
human	WB	Life Sci; Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway; Authors - Jia-Peng Lin
human,rat	WB,IHC	Am J Physiol Lung Cell Mol Physiol; KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension; Authors - Xiaochen Li
human	WB	Cancer Cell Int; Inhibition of hyaluronic acid degradation pathway suppresses glioma progression by inducing apoptosis and cell cycle arrest; Authors - Tao Yan