Anticorps Recombinant de lapin anti-Cyclin B1

Cyclin B1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250227C12

N° de cat : 85895-3-RR

Synonymes

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

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Galerie de données de validation

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  WB analysis using 85895-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85895-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85895-3-RR (CCNB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85895-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85895-3-RR against Human Cyclin B1 were performed. The affinity constant is 2.70 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85895-3-RR cible Cyclin B1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cyclin B1 Protéine recombinante Ag29426
• Nom complet: cyclin B1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC006510
• Symbole du gène: Cyclin B1
• Identification du gène (NCBI): 891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

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