Anticorps Recombinant de lapin anti-Cyclin D1

Cyclin D1 Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6B10

N° de cat : 82681-1-PBS

Synonymes

CCND1, 6B10, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL-1

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Galerie de données de validation

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  WB analysis using 82681-1-RR (same clone as 82681-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82681-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP80003-1

  Sandwich ELISA standard curve of MP80003-1, Cyclin D1 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 68790-1-PBS. Detection antibody: 82681-1-PBS. Standard: Ag25384. Range: 0.188-12 ng/mL.

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  IHC staining of human oesophagus cancer using 82681-1-RR (same clone as 82681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82681-1-RR (same clone as 82681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82681-1-RR (Cyclin D1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82681-1-RR (same clone as 82681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (82681-1-RR, Clone: 6B10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82681-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82681-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82681-1-RR against Human Cyclin D1 were performed. The affinity constant is 0.153 nM.

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Informations sur le produit

82681-1-PBS cible Cyclin D1 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cyclin D1 Protéine recombinante Ag25384
• Nom complet: cyclin D1
• Masse moléculaire calculée: 295 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000076
• Symbole du gène: Cyclin D1
• Identification du gène (NCBI): 595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

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