Anticorps Monoclonal anti-Cyclin D1

• Phare
• Validé par KD/KO

Cyclin D1 Monoclonal Antibody for WB, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra)

Conjugaison

Cardinal Red™ Fluorescent Dye

CloneNo.

2G3G5

N° de cat : CR-60186

Synonymes

CCND1, BCL-1 oncogene, BCL-1, B-cell lymphoma 1 protein, B cell lymphoma 1 protein

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Galerie de données de validation

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  WB analysis using CR-60186

  Various lysates were subjected to SDS PAGE followed by western blot with CR-60186 (Cyclin D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CR-60186

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human Cyclin D1 (CR-60186, Clone:2G3G5) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HeLa, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CR-60186 cible Cyclin D1 dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin D1 Protéine recombinante Ag0689
• Nom complet: cyclin D1
• Masse moléculaire calculée: 295 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000076
• Symbole du gène: Cyclin D1
• Identification du gène (NCBI): 595
• Conjugaison: Cardinal Red™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 592 nm / 611 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

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