Anticorps Monoclonal anti-Cyclin D3

• Phare
• Validé par KD/KO

Cyclin D3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F2C6

N° de cat : 66357-1-PBS

Synonymes

CCND3, 1F2C6, Cyclin D, G1/S specific cyclin D3, G1/S-specific cyclin-D3

---

Galerie de données de validation

• 

  WB analysis of Raji using 66357-1-Ig (same clone as 66357-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66357-1-Ig (same clone as 66357-1-PBS)

  WB result of Cyclin D3 antibody (66357-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66357-1-Ig (same clone as 66357-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66357-1-Ig (same clone as 66357-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66357-1-Ig (same clone as 66357-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 66357-1-Ig (same clone as 66357-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66357-1-Ig (same clone as 66357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66357-1-Ig (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66357-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66357-1-PBS cible Cyclin D3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin D3 Protéine recombinante Ag22825
• Nom complet: cyclin D3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC011616
• Symbole du gène: Cyclin D3
• Identification du gène (NCBI): 896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

{{ptg:RelatedPrimaryAntibodies}}