Anticorps Recombinant de lapin anti-Cyclin E1

Cyclin E1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240136G9

N° de cat : 82700-8-PBS

Synonymes

CCNE1, 240136G9, CCNE, Cyclin E, G1/S specific cyclin E1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00361-2

  Sandwich ELISA standard curve of MP00361-2, Human Cyclin E1 Monoclonal Matched Antibody Pair - PBS only. 82700-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2110. 82700-8-PBS was HRP conjugated as the detection antibody. Range: 0.313-40 ng/mL.

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  Sample test of MP00361-2

  The mean human Cyclin E1 concentration was determined to be 132.4 ng/mL in Hela cell extract based on a 3.0 mg/mL extract load.

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  IF Staining of HepG2 using 82700-8-RR (same clone as 82700-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82700-8-RR (same clone as 82700-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E1 antibody (82700-8-RR, Clone: 240136G9 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82700-8-RR (same clone as 82700-8-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82700-8-RR (same clone as 82700-8-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant antibody (82700-8-RR, Clone:240136G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-8-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00361-2

  Cytometric bead array standard curve of MP00361-2, Cyclin E1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82700-6-PBS. Detection antibody: 82700-8-PBS. Standard: Ag2110. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 82700-8-PBS

  Biolayer interferometry (BLl) kinetic assay of 82700-8-PBS against Human Cyclin E1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 82700-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-8-RR against Human Cyclin E1 were performed. The affinity constant is 0.229 nM.

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Informations sur le produit

82700-8-PBS cible Cyclin E1 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cyclin E1 Protéine recombinante Ag2110
• Nom complet: cyclin E1
• Masse moléculaire calculée: 410 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC035498
• Symbole du gène: Cyclin E1
• Identification du gène (NCBI): 898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

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