Anticorps Recombinant de lapin anti-Cyclin E1

Cyclin E1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240136H5

N° de cat : 82700-9-PBS

Synonymes

CCNE1, 240136H5, CCNE, Cyclin E, G1/S specific cyclin E1

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Galerie de données de validation

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  IHC staining of rat testis using 82700-9-RR (same clone as 82700-9-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 82700-9-RR (same clone as 82700-9-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82700-9-RR (same clone as 82700-9-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82700-9-RR (same clone as 82700-9-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82700-9-RR (same clone as 82700-9-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82700-9-RR (same clone as 82700-9-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82700-9-RR (same clone as 82700-9-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82700-9-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82700-9-PBS

  Biolayer interferometry (BLl) kinetic assay of 82700-9-PBS against Human Cyclin E1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 82700-9-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-9 -RR against Human Cyclin E were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82700-9-PBS cible Cyclin E1 dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cyclin E1 Protéine recombinante Ag2110
• Nom complet: cyclin E1
• Masse moléculaire calculée: 410 aa, 47 kDa
• Numéro d’acquisition GenBank: BC035498
• Symbole du gène: Cyclin E1
• Identification du gène (NCBI): 898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

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