Anticorps Recombinant de lapin anti-Cyclin E1

Cyclin E1 Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240136G9

N° de cat : CL488-82700-8

Synonymes

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-82700-8

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Cyclin E antibody (CL488-82700-8, Clone: 240136G9 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-82700-8

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Cyclin E1 Recombinant antibody (CL488-82700-8, Clone:240136G9)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of MCF-7 using CL488-82700-8

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Cyclin E1 Recombinant antibody (CL488-82700-8, Clone:240136G9)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa, cellules MCF-7

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-82700-8 cible Cyclin E1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cyclin E1 Protéine recombinante Ag2110
• Nom complet: cyclin E1
• Masse moléculaire calculée: 410 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC035498
• Symbole du gène: Cyclin E1
• Identification du gène (NCBI): 898
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

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