Anticorps Monoclonal anti-Cyclin H

Cyclin H Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C5G11

N° de cat : 67065-1-PBS

Synonymes

CCNH, 2C5G11, p34, MO15-associated protein, MO15 associated protein

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Galerie de données de validation

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  WB analysis using 67065-1-Ig (same clone as 67065-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  WB analysis using 67065-1-Ig (same clone as 67065-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67065-1-Ig (Cyclin H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67065-1-Ig (same clone as 67065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67065-1-Ig (same clone as 67065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67065-1-Ig (Cyclin H antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 67065-1-Ig (same clone as 67065-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Cyclin H antibody (67065-1-Ig, Clone: 2C5G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 67065-1-Ig (same clone as 67065-1-PBS)

  1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin H (67065-1-Ig, Clone:2C5G11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67065-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67065-1-PBS cible Cyclin H dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cyclin H Protéine recombinante Ag28556
• Nom complet: cyclin H
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC005280
• Symbole du gène: Cyclin H
• Identification du gène (NCBI): 902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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