Anticorps Polyclonal de lapin anti-Cyclin T1

Cyclin T1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20992-1-PBS

Synonymes

CCNT1, CCNT, CYCT1, Cyclin-T1, Cyclin-T

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Galerie de données de validation

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  WB analysis using 20992-1-AP (same clone as 20992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 20992-1-AP (same clone as 20992-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 20992-1-AP (same clone as 20992-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20992-1-AP (same clone as 20992-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 20992-1-AP (same clone as 20992-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20992-1-AP (same clone as 20992-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20992-1-AP (same clone as 20992-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20992-1-AP (Cyclin T1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 20992-1-AP (same clone as 20992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20992-1-AP (Cyclin T1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20992-1-AP (same clone as 20992-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin T1 antibody (20992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20992-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20992-1-PBS cible Cyclin T1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cyclin T1
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: NM_001240
• Symbole du gène: Cyclin T1
• Identification du gène (NCBI): 904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCNT1, also named as CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

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