Anticorps Recombinant de lapin anti-Cyclin T1

Cyclin T1 Recombinant Antibody for WB, FC (Intra), ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

242256C3

N° de cat : 84980-1-RR

Synonymes

CCNT1, 242256C3, CCNT, Cyclin T, Cyclin-T

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Galerie de données de validation

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  WB analysis using 84980-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84980-1-RR (Cyclin T1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 84980-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of U2OS using 84980-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Cyclin T1 Recombinant antibody (84980-1-RR, Clone:242256C3) (red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of HCT 116 using 84980-1-RR

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Cyclin T1 (84980-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7
• Résultats positifs en FC (Intra): cellules MCF-7, cellules U2OS
• Résultats positifs en ChIP-qPCR: cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84980-1-RR cible Cyclin T1 dans les applications de WB, FC (Intra), ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cyclin T1
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 75-81 kDa
• Numéro d’acquisition GenBank: NM_001240
• Symbole du gène: Cyclin T1
• Identification du gène (NCBI): 904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCNT1, also named as Cyclin T1 and CycT1, belongs to the cyclin family and Cyclin C subfamily. It is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex which also called positive transcription elongation factor B (P-TEFb). In case of HIV or SIV infections, CCNT1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). CCNT1 serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. The antibody is specific to CCNT1.

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