Anticorps Polyclonal de lapin anti-Cyclon/CCDC86

Cyclon/CCDC86 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14947-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse stomach using 14947-1-AP (same clone as 14947-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14947-1-AP (same clone as 14947-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 14947-1-AP (same clone as 14947-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14947-1-AP (same clone as 14947-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human stomach using 14947-1-AP (same clone as 14947-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14947-1-AP (Cyclon/CCDC86 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 14947-1-AP (same clone as 14947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cyclon/CCDC86 antibody (14947-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14947-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14947-1-PBS cible Cyclon/CCDC86 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclon/CCDC86 Protéine recombinante Ag6754
• Nom complet: coiled-coil domain containing 86
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC001378
• Symbole du gène: CCDC86
• Identification du gène (NCBI): 79080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCDC86 (Coiled-coil domain-containing protein 86), also known as Cyclon (cytokine-induced protein with coiled-coil domain) ) is known for its expression in leukocytes in mice, where it regulates the immune response. Cyclon/CCDC86 regulates the apoptosis of T-cells upon activation, a process known as an activationinduced cellular death (AICD), by regulating the expression of the death receptor Fas on T-cells (PMID: 23439384). CCDC86 depletion led to a novel phenotype in which Ki-67 and nucleolin could still accumulate at the chromosome periphery but also formed abnormal cytoplasmic NDF-like foci (PMID: 36695333). The detected molecular weights (MWs) of Cyclon on SDS-PAGE (human Cyclon: 65 kDa; mouse Cyclon: 75 kDa) were higher than calculated MWs (human Cyclon: 40.2 kDa; mouse Cyclon: 46.5 kDa), suggesting possible modifications of Cyclon (PMID: 17300783).

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