Anticorps Recombinant de lapin anti-Cystatin C

Cystatin C Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240202A2

N° de cat : 83422-1-PBS

Synonymes

Cst3, CysC, 240202A2

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Galerie de données de validation

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  WB analysis using 83422-1-RR (same clone as 83422-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83422-1-RR (Cystatin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83422-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 83422-1-RR (same clone as 83422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83422-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 83422-1-RR (same clone as 83422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83422-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83422-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83422-1-RR against Mouse Cystatin C were performed. The affinity constant is 20.3 nM.

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Informations sur le produit

83422-1-PBS cible Cystatin C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cystatin C
• Masse moléculaire calculée: 15KD
• Poids moléculaire observé: 13-16 kDa
• Numéro d’acquisition GenBank: NM_009976.4
• Symbole du gène: Cystatin C
• Identification du gène (NCBI): 13010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney.

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