Anticorps Recombinant de lapin anti-Cystatin C

Cystatin C Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240202A2

N° de cat : 83422-1-RR

Synonymes

Cst3, CysC, 240202A2

---

Galerie de données de validation

• 

  WB analysis using 83422-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83422-1-RR (cystatin c antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 83422-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 83422-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83422-1-RR (cystatin c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83422-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83422-1-RR against Mouse Cystatin C were performed. The affinity constant is 20.3 nM.

---

Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83422-1-RR cible Cystatin C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cystatin C
• Masse moléculaire calculée: 15KD
• Poids moléculaire observé: 13-16 kDa
• Numéro d’acquisition GenBank: NM_009976.4
• Symbole du gène: Cystatin C
• Identification du gène (NCBI): 13010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidney.

{{ptg:RelatedPrimaryAntibodies}}