Anticorps Recombinant de lapin anti-Cystatin SN/CST1

Cystatin SN/CST1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242888B3

N° de cat : 85403-1-RR

Synonymes

CST1, Cystain SA I, Cystain-SA-I, Cystatin 1, cystatin SN

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Galerie de données de validation

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  WB analysis using 85403-1-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 85403-1-RR (CST1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 85403-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 85403-1-RR (Cystatin SN/CST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85403-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85403-1-RR against Human Cystatin SN/CST1 were performed. The affinity constant is 1.09 nM.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, salive humain, salive humaine

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85403-1-RR cible Cystatin SN/CST1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cystatin SN/CST1 Protéine recombinante Ag8862
• Nom complet: cystatin SN
• Masse moléculaire calculée: 141 aa, 16 kDa
• Poids moléculaire observé: 14-16 kDa
• Numéro d’acquisition GenBank: BC021225
• Symbole du gène: CST1
• Identification du gène (NCBI): 1469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.

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