Anticorps Monoclonal anti-Cytochrome c

• Phare
• Validé par KD/KO

Cytochrome c Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D8D11

N° de cat : 66264-1-PBS

Synonymes

CYCS, CYC, 2D8D11

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Galerie de données de validation

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  WB analysis using 66264-1-Ig (same clone as 66264-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66264-1-Ig (same clone as 66264-1-PBS)

  WB result of Cytochrome c antibody (66264-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome c transfected HepG2 cells. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66264-1-Ig (same clone as 66264-1-PBS)

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66264-1-Ig (same clone as 66264-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66264-1-Ig (same clone as 66264-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skeletal muscle using 66264-1-Ig (same clone as 66264-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 66264-1-Ig (same clone as 66264-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66264-1-Ig (Cytochrome c Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66264-1-Ig (Cytochrome c antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66264-1-Ig (same clone as 66264-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cytochrome c antibody (66264-1-Ig, Clone: 2D8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66264-1-Ig (same clone as 66264-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Cytochrome c (66264-1-Ig, Clone:2D8D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66264-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66264-1-PBS cible Cytochrome c dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytochrome c Protéine recombinante Ag24349
• Nom complet: cytochrome c, somatic
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12-15 kDa
• Numéro d’acquisition GenBank: BC009578
• Symbole du gène: Cytochrome c
• Identification du gène (NCBI): 54205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

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