Anticorps Monoclonal anti-Cytohesin 2

• Phare
• Validé par KD/KO

Cytohesin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A7A1

N° de cat : 67185-1-PBS

Synonymes

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like

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Galerie de données de validation

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  WB analysis using 67185-1-Ig (same clone as 67185-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67185-1-Ig (CYTH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67185-1-Ig (same clone as 67185-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67185-1-Ig (same clone as 67185-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67185-1-Ig (same clone as 67185-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67185-1-Ig (same clone as 67185-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67185-1-Ig (Cytohesin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67185-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67185-1-PBS cible Cytohesin 2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytohesin 2 Protéine recombinante Ag21279
• Nom complet: cytohesin 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC004361
• Symbole du gène: Cytohesin 2
• Identification du gène (NCBI): 9266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.

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