Anticorps Recombinant de lapin anti-Cytokeratin 10

Cytokeratin 10 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

250893H2

N° de cat : 86324-1-RR

Synonymes

KRT10, CK 10, CK10, CK-10, Cytokeratin,Keratin

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Galerie de données de validation

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  WB analysis using 86324-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86324-1-RR (KRT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 86324-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 86324-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 86324-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 86324-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, tissu cutané de rat
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cutané de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86324-1-RR cible Cytokeratin 10 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 10
• Masse moléculaire calculée: 584 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC034697
• Symbole du gène: Cytokeratin 10
• Identification du gène (NCBI): 3858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 10 (CK-10, K10, KRT10) is a type I (acidic) cytokeratin and part of the intermediate filament protein superfamily. It is expressed in keratinized stratified epithelium, suprabasal cell layers (including the stratum corneum), and differentiated areas of highly differentiated squamous cell carcinomas. CK-10 plays a role in maintaining the structural integrity of epithelial cells and is involved in differentiation, tissue specialization, and epidermal barrier establishment. Mutations in the KRT10 gene are associated with epidermolytic hyperkeratosis, and its expression is linked to the terminal differentiation of epidermal cells . CK-10 is also used as a marker for tissue differentiation and characterization of malignant tumors in surgical pathology.

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