Anticorps Monoclonal anti-Cytokeratin 13

• Phare
• Validé par KD/KO

Cytokeratin 13 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E10G2

N° de cat : 66684-1-PBS

Synonymes

KRT13, 1E10G2, CK 13, CK13, CK-13

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Galerie de données de validation

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  WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

  WB result of Cytokeratin 13 antibody (66684-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skin using 66684-1-Ig (same clone as 66684-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51141-1

  Cytometric bead array standard curve of MP51141-1, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-2-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP51141-3

  Cytometric bead array standard curve of MP51141-3, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-4-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

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  Affinity and Kinetic Characterization of 66684-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66684-1-Ig against Human Cytokeratin 13 was performed. The affinity constant is below 1 pM.

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Informations sur le produit

66684-1-PBS cible Cytokeratin 13 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 13 Protéine recombinante Ag0217
• Nom complet: keratin 13
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC002661
• Symbole du gène: Cytokeratin 13
• Identification du gène (NCBI): 3860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.

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