Anticorps Recombinant de lapin anti-Cytokeratin 14

Cytokeratin 14 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1H22

N° de cat : 83379-1-RR

Synonymes

KRT14, 1H22, CK 14, CK14, CK-14

---

Galerie de données de validation

• 

  WB analysis of rat skin using 83379-1-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83379-1-RR (KRT14 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 83379-1-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83379-1-RR (Cytokeratin 14 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 83379-1-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83379-1-RR (Cytokeratin 14 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 83379-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 14 antibody (83379-1-RR, Clone: 1H22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83379-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83379-1-RR against Human Cytokeratin 14 were performed. The affinity constant is 43.1 nM.

---

Applications testées

• Résultats positifs en WB: tissu cutané de rat,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cutané de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2800
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83379-1-RR cible Cytokeratin 14 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 14 Protéine recombinante Ag0188
• Nom complet: keratin 14
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC002690
• Symbole du gène: Cytokeratin 14
• Identification du gène (NCBI): 3861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 14, one of about 20 different cytokeratin isotypes of human cells, is the intermediate filament protein characteristic of epithelial cells. Cytokeratin 14 is expressed in the basal compartment of all stratified squamous epithelia. In various kinds of human tumors, the appearance and increasing expression of Cytokeratin 14 were strikingly associated with higher grade and stage of carcinoma, with varying degrees of unfavorable prognosis. In lung squamous cell carcinoma(LSCC), Cytokeratin 14 was expressed in the tumor cell nests showing stromal invasion with fibrosis and lymph node metastases, indicating that Cytokeratin 14 involved in proliferation and metastasis of LSCC. Cytokeratin 14 expression is sometimes used in diagnosis of myoepithelioma1 and intraductal vs. invasive ductal carcinoma of the breast.

{{ptg:RelatedPrimaryAntibodies}}