Anticorps Monoclonal anti-Cytokeratin 14

Cytokeratin 14 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF

Conjugaison

CoraLite®488 Fluorescent Dye

CloneNo.

2G1E2

N° de cat : CL488-60320

Synonymes

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

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Galerie de données de validation

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  IF Staining of A431 using CL488-60320

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CL488-60320 (Cytokeratin 14 antibody) at dilution of 1:100.

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  IF Staining of HaCaT using CL488-60320

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

• 

  IF Staining of mouse skin using CL488-60320

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

• 

  IF Staining of mouse skin using CL488-60320

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

• 

  IF Staining of A431 using CL488-60320

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-60320

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®488 Cytokeratin 14 antibody (CL488-60320, Clone: 2G1E2 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF: cellules A431, cellules HaCaT, cellules HepG2, tissu cutané de souris

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60320 cible Cytokeratin 14 dans les applications de IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 14 Protéine recombinante Ag17559
• Nom complet: keratin 14
• Masse moléculaire calculée: 472 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC002690
• Symbole du gène: KRT14
• Identification du gène (NCBI): 3861
• Conjugaison: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

eratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.