Anticorps Monoclonal anti-Cytokeratin 15

Cytokeratin 15 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6B4F8

N° de cat : 60247-1-PBS

Synonymes

KRT15, 6B4F8, CK 15, CK15, CK-15

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Galerie de données de validation

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  WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50002-1

  Sandwich ELISA standard curve of MP50002-1, KRT15/Cytokeratin 15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: HRP-conjugated 60247-2-PBS. Standard: Ag0185. Range: 0.313-20 ng/mL.

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  IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50002-1

  Cytometric bead array standard curve of MP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: 60247-2-PBS. Standard: Ag0185. Range: 1.563-100 ng/mL.

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Informations sur le produit

60247-1-PBS cible Cytokeratin 15 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 15 Protéine recombinante Ag0185
• Nom complet: keratin 15
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC002641
• Symbole du gène: KRT15
• Identification du gène (NCBI): 3866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

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