Anticorps Monoclonal anti-Cytokeratin 16

• Phare
• Validé par KD/KO

Cytokeratin 16 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2H4D8

N° de cat : 66802-1-Ig

Synonymes

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

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Galerie de données de validation

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  WB analysis of A431 using 66802-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66802-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human milk using 66802-1-Ig

  human milk were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66802-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (66802-1-Ig, Clone: 2H4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of A431 using 66802-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 16 (66802-1-Ig, Clone:2H4D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, lait humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la peau humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66802-1-Ig cible Cytokeratin 16 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 16 Protéine recombinante Ag11240
• Nom complet: keratin 16
• Masse moléculaire calculée: 473 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC039169
• Symbole du gène: KRT16
• Identification du gène (NCBI): 3868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Publications

Species	Application	Title
human	WB,IHC,IF	Cancers (Basel); Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.; Authors - Maha Elazezy ; KD Validated
human	WB,IHC	Nat Commun; Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer; Authors - Xuejiao Liu

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C