Anticorps Monoclonal anti-Cytokeratin 16

• Phare
• Validé par KD/KO

Cytokeratin 16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H4D8

N° de cat : 66802-1-PBS

Synonymes

KRT16, 2H4D8, CK 16, CK16, CK-16

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Galerie de données de validation

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  WB analysis of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66802-1-Ig (same clone as 66802-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  WB analysis of human milk using 66802-1-Ig (same clone as 66802-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66802-1-Ig (Cytokeratin 16 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66802-1-Ig (same clone as 66802-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (66802-1-Ig, Clone: 2H4D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66802-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66802-1-PBS cible Cytokeratin 16 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 16 Protéine recombinante Ag11240
• Nom complet: keratin 16
• Masse moléculaire calculée: 473 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC039169
• Symbole du gène: Cytokeratin 16
• Identification du gène (NCBI): 3868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

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