Anticorps Monoclonal anti-Cytokeratin 18

• Phare
• Validé par KD/KO

Cytokeratin 18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G11C4

N° de cat : 66187-1-PBS

Synonymes

KRT18, 1G11C4, Cell proliferation-inducing gene 46 protein, CK 18, CK18

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Galerie de données de validation

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  WB analysis using 66187-1-Ig (same clone as 66187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  WB result of Cytokeratin 18 antibody (66187-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 18 transfected HeLa cells. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skin using 66187-1-Ig (same clone as 66187-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66187-1-Ig (Cytokeratin 18 antibody at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66187-1-Ig (Cytokeratin 18 antibody) at dilution of 1:2400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 66187-1-Ig (same clone as 66187-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using Cytokeratin 18 antibody (66187-1-Ig, Clone: 1G11C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66187-1-Ig (same clone as 66187-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 18 (66187-1-Ig, Clone:1G11C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66187-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50238-1

  Cytometric bead array standard curve of MP50238-1, KRT18 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66187-2-PBS. Detection antibody: 66187-1-PBS. Standard:Ag1260. Range: 0.391-100 ng/mL.

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Informations sur le produit

66187-1-PBS cible Cytokeratin 18 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 18 Protéine recombinante Ag1260
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: Cytokeratin 18
• Identification du gène (NCBI): 3875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

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