Anticorps Recombinant de lapin anti-Cytokeratin 18

• Phare
• Validé par KD/KO

Cytokeratin 18 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F2

N° de cat : 82817-5-PBS

Synonymes

KRT18, 4F2, Cell proliferation-inducing gene 46 protein, CK 18, CK18

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Galerie de données de validation

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  WB analysis of HeLa using 82817-5-RR (same clone as 82817-5-PBS)

  WB result of Cytokeratin 18 antibody (82817-5-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 18 transfected HeLa cells. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  WB analysis using 82817-5-RR (same clone as 82817-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 82817-5-RR (same clone as 82817-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82817-5-RR (same clone as 82817-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 18 antibody (82817-5-RR, Clone: 4F2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82817-5-PBS in a different storage buffer formulation.

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Informations sur le produit

82817-5-PBS cible Cytokeratin 18 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 18 Protéine recombinante Ag1260
• Nom complet: keratin 18
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000180
• Symbole du gène: Cytokeratin 18
• Identification du gène (NCBI): 3875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.

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