Anticorps Monoclonal anti-Cytokeratin 19

• Phare
• Validé par KD/KO

Cytokeratin 19 Monoclonal Antibody for WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G1E4

N° de cat : 60187-1-PBS

Synonymes

CK 19, CK19, KRT19, 3G1E4, CK-19

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Galerie de données de validation

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  WB analysis of MCF-7 using 60187-1-Ig (same clone as 60187-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60187-1-Ig (same clone as 60187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60187-1-Ig (same clone as 60187-1-PBS)

  WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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  WB analysis of HaCaT using 60187-1-Ig (same clone as 60187-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 60187-1-Ig (same clone as 60187-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:25000 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:25000 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse breast cancer using 60187-1-Ig (same clone as 60187-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 60187-1-Ig (same clone as 60187-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60187-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60187-1-PBS cible Cytokeratin 19 dans les applications de WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 19 Protéine recombinante Ag7407
• Nom complet: keratin 19
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC002539
• Symbole du gène: Cytokeratin 19
• Identification du gène (NCBI): 3880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.

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