Anticorps Recombinant de lapin anti-Cytokeratin 19

Cytokeratin 19 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250894B1

N° de cat : 86250-1-PBS

Synonymes

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

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Galerie de données de validation

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  WB analysis using 86250-1-RR (same clone as 86250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 86250-1-RR (same clone as 86250-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cytokeratin 19 Recombinant antibody (86250-1-RR, Clone:250894B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86250-1-PBS cible Cytokeratin 19 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 19
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC002539
• Symbole du gène: Cytokeratin 19
• Identification du gène (NCBI): 3880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.

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