Anticorps Recombinant de lapin anti-Cytokeratin 20

Cytokeratin 20 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H10

N° de cat : 82428-1-PBS

Synonymes

KRT20, CK 20, CK20, CK-20, Cytokeratin-20

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Galerie de données de validation

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  WB analysis using 82428-1-RR (same clone as 82428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 82428-1-RR (same clone as 82428-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82428-1-PBS cible Cytokeratin 20 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 20 Protéine recombinante Ag11308
• Nom complet: keratin 20
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC031559
• Symbole du gène: Cytokeratin 20
• Identification du gène (NCBI): 54474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

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