Anticorps Monoclonal anti-Cytokeratin 20

Cytokeratin 20 Monoclonal Antibody for IF/ICC, IF-P

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF/ICC, IF-P

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

4D10A4

N° de cat : CL555-60183

Synonymes

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

---

Galerie de données de validation

• 

  IF Staining of human colon using CL555-60183

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®555 Cytokeratin 20 antibody (CL555-60183, Clone: 4D10A4 ) at dilution of 1:200.

• 

  IF Staining of human colon using CL555-60183

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®555 Cytokeratin 20 antibody (CL555-60183, Clone: 4D10A4 ) at dilution of 1:200.

• 

  IF Staining of HT-29 using CL555-60183

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite®555 Cytokeratin 20 antibody (CL555-60183, Clone: 4D10A4 ) at dilution of 1:200.

• 

  IF Staining of human colon cancer using CL555-60183

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 Cytokeratin 20 antibody (CL555-60183, Clone: 4D10A4 ) at dilution of 1:200.

• 

  IF Staining of human colon cancer using CL555-60183

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 Cytokeratin 20 antibody (CL555-60183, Clone: 4D10A4 ) at dilution of 1:200.

---

Applications testées

• Résultats positifs en IF-P: tissu de côlon humain,
• Résultats positifs en IF/ICC: cellules HT-29, tissu de cancer du côlon humain

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-60183 cible Cytokeratin 20 dans les applications de IF/ICC, IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 20 Protéine recombinante Ag11336
• Nom complet: keratin 20
• Masse moléculaire calculée: 424 aa, 48 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC031559
• Symbole du gène: Cytokeratin 20
• Identification du gène (NCBI): 54474
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

{{ptg:RelatedPrimaryAntibodies}}