Anticorps Recombinant de lapin anti-Cytokeratin 39

Cytokeratin 39 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

250891F5

N° de cat : 86277-2-RR

Synonymes

CK-39, Cytokeratin-39, K39, KA35, Keratin, type I cytoskeletal 39

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Galerie de données de validation

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  WB analysis using 86277-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86277-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse esophagus using 86277-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse esophagus using 86277-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 86277-2-RR

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 86277-2-RR

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 86277-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 86277-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 86277-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 86277-2-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules A431, cellules HaCaT, cellules HeLa, cellules MCF-7, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IHC: mouse esophagus tissue, tissu cutané de souris, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cutané de souris,
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86277-2-RR cible Cytokeratin 39 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 39
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC157887
• Symbole du gène: KRT39
• Identification du gène (NCBI): 390792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 39 (CK-39) is a member of the cytokeratin family, a group of intermediate filament proteins primarily expressed in epithelial cells. Cytokeratins are classified into Type I (acidic) and Type II (basic) subfamilies, which pair to form heteropolymers essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. CK-39 is predominantly expressed in stratified and glandular epithelia, such as those found in the prostate, bladder, and respiratory tract. Its expression pattern may vary depending on tissue differentiation states.

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