Anticorps Polyclonal de lapin anti-Cytokeratin 4

Cytokeratin 4 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16572-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of A431 using 16572-1-AP (same clone as 16572-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  WB analysis of human bladder using 16572-1-AP (same clone as 16572-1-PBS)

  human bladder tissue were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 16572-1-AP (same clone as 16572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 16572-1-AP (same clone as 16572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 16572-1-AP (Cytokeratin 4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 16572-1-AP (same clone as 16572-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 16572-1-AP (same clone as 16572-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16572-1-PBS cible Cytokeratin 4 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 4 Protéine recombinante Ag9854
• Nom complet: keratin 4
• Masse moléculaire calculée: 534 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC042174
• Symbole du gène: Cytokeratin 4
• Identification du gène (NCBI): 3851
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

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