Anticorps Polyclonal de lapin anti-Cytokeratin 5

Cytokeratin 5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 28506-1-AP

Synonymes

CK 5, CK-5, keratin 5, KRT5, Cytokeratin5

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Galerie de données de validation

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  WB analysis using 28506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brown disease using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 28506-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, tissus cutanés de rat, tissus cutanés de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 7 publications below

Informations sur le produit

28506-1-AP cible Cytokeratin 5 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 5 Protéine recombinante Ag25436
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: Cytokeratin 5
• Identification du gène (NCBI): 3852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells

Publications

Species	Application	Title
human	IF	Stem Cell Res Ther; Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2; Authors - Zhilin Sui
human	IF	J Invest Dermatol; Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo.; Authors - Li Dong
mouse	IF	FASEB J; Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia; Authors - Sheng Gao
human	WB	J Clin Lab Anal; Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis.; Authors - Shan Chen
mouse	IF	J Oral Biosci; Epigenetic modifier G9a is involved in regulation of mouse tongue development; Authors - Hisashi Ideno
human	IF	Cancer Lett; The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma; Authors - Zhe Yi Wang

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