Anticorps Monoclonal anti-Cytokeratin 5

Cytokeratin 5 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1A1C5

N° de cat : 66727-1-PBS

Synonymes

CK 5, CK-5, keratin 5, KRT5, 1A1C5

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Galerie de données de validation

• 

  WB analysis using 66727-1-Ig (same clone as 66727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 66727-1-Ig (same clone as 66727-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SKOV-3 using 66727-1-Ig (same clone as 66727-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66727-1-Ig (same clone as 66727-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66727-1-Ig (same clone as 66727-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 66727-1-Ig (same clone as 66727-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 10x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66727-1-Ig (Cytokeratin 5 antibody) at dilution of 1:10000 (under 40x lens) showing negative staining. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human oesophagus cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human oesophagus cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66727-1-Ig (same clone as 66727-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (66727-1-Ig, Clone: 1A1C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66727-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66727-1-PBS cible Cytokeratin 5 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 5 Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: Cytokeratin 5
• Identification du gène (NCBI): 3852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD keratin 14 in stratified squamous epithelia. Keratin 5, one 58-kD type II keratin, is coexpressed with a 50-kD tyK14 in stratified squamous epithelia.

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