Anticorps Monoclonal anti-Cytokeratin 6A

Cytokeratin 6A Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H6A11

N° de cat : 66685-1-PBS

Synonymes

Cytokeratin 6, KRT6A, 2H6A11, CK 6A, CK6

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Galerie de données de validation

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  WB analysis of A431 using 66685-1-Ig (same clone as 66685-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66685-1-Ig (KRT6A antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66685-1-Ig (same clone as 66685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66685-1-Ig (same clone as 66685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66685-1-Ig (same clone as 66685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66685-1-Ig (same clone as 66685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66685-1-Ig (Cytokeratin 6A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66685-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50382-1

  Cytometric bead array standard curve of MP50382-1, KRT6A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66685-1-PBS. Detection antibody: 68294-2-PBS. Standard:Ag0882. Range: 0.098-100 ng/mL.

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Informations sur le produit

66685-1-PBS cible Cytokeratin 6A dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 6A Protéine recombinante Ag0882
• Nom complet: keratin 6A
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC008807
• Symbole du gène: Cytokeratin 6A
• Identification du gène (NCBI): 3853
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three are three keratin 6 isoforms: ketatin 6A, 6B and 6C. They share more than 99% identical DNA sequence.

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