Anticorps Polyclonal de lapin anti-Cytokeratin 6B

• Phare
• Validé par KD/KO

Cytokeratin 6B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17391-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse skin using 17391-1-AP (same clone as 17391-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 17391-1-AP (same clone as 17391-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skin using 17391-1-AP (same clone as 17391-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 17391-1-AP (same clone as 17391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17391-1-AP (Cytokeratin 6B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 6B antibody (17391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 17391-1-AP (same clone as 17391-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 6B (17391-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17391-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17391-1-PBS cible Cytokeratin 6B dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 6B Protéine recombinante Ag11288
• Nom complet: keratin 6B
• Masse moléculaire calculée: 564 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC034535
• Symbole du gène: Cytokeratin 6B
• Identification du gène (NCBI): 3854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as a marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B, and 6C. They share more than 99% identical DNA sequence.

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